Miyamae Kanako, a high school girl, who is allergic to boys, enrols in an all-girls school, hoping to find a female romantic partner. However, her ideal candidate, Shidou Mariya, turns out to be a mischievous cross-dressing boy, who together with his sarcastic maid Shinouji Matsurika, give Kanako really difficult times. 
Time passes and Kanako still fantasizes about her ideal female romantic partner(s), to no avail, alas. Living in the same room at the dormitory Mariya and his outspoken maid keep on challenging Kanako's sense of reality on a daily basis. 
(Source: AniDB)

It’s not easy to find the perfect girl, even when you’re the same sex! Kanako thought attending an all girls school would be the perfect way to meet the girl of her dreams, but after her hot new roommate turns out to be a guy in drag, and catching the eye of the most popular girl in school only brings down the wrath of all the OTHER similarly obsessed girls, she’s not so sure! Complicate things with a cat-eared dorm leader, her roommate’s rude-mannered maid and identical twin sister (who attends an all boys school, of course,) and WAY too many attractive girls of questionable persuasions and the question becomes whether Kanako can die from frustrated nosebleeds! (Life would be SO much easier if guys didn’t literally give her the hives!) Will Kanako ever find a non-polar opposite of a compatible non-opposing polarity? 
 
(Source: Sentai Filmworks)

Kanako is excited to be transferring into a new all-girl mission school. She’s looking for her ideal yuri partner. When she meets Mariya Shidou she thinks she’s found the one. But wait! Mariya is a cross-dressing sadistic boy and now Kanako is forced to keep his secret! But that’s not all. To complicate things she’s drawing the attention of the most popular girl in school and, at the same time, catching the wrath of all the other girls. Will Kanako ever find the cute girl of her dreams? Or will she die of sexual-frustration nosebleeds? Love is a complicated affair for Kanako and the girls of Maria Holic. 
(Source: One Peace Books)

An unaired scene (C part) from the first episode.

The Maria Holic's official website put this video on its homepage on Children's Day.

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